Product Name	Recombinant Rabbit Anti-TNNI3 Monoclonal Antibody
Cat No.	SLA1959M
Chinese Name	心肌肌钙蛋白重组兔单抗
Alias	TNNC1_HUMAN; TNNC1; TN-C; TNNC; CMD1Z; CMH13; TNC; troponin C1, slow skeletal and cardiac type; troponin C type 1 (slow)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11E5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human TNNC1
Purification	affinity purified by Protein A
Predicted MW.	18 kDa
Observed MW.	18 kDa
Gene ID	7134
Swiss-Prot	P63316
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with TNNI3 monoclonal antibody, unconjugated (SLA1959M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo (heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (SLA1959M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Embryo (heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (SLA1959M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (SLA1959M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (SLA1959M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (SLA1959M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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