Product Name	Recombinant Rabbit Anti-CTBP1 Monoclonal Antibody
Cat No.	SLA1957M
Chinese Name	C端结合蛋白1重组兔单抗
Alias	BARS; HADDTS; CtBP3/BARS; D4S115h; D5H4S115; D5H4S115E; 50-kDaBFA-inducedADP-ribosylatedsubstrate; BARS-50; CTBP1_HUMAN; CTBP1; 1.1.1.-; CTBP; CTBP1_MOUSE; CTBP1_RAT; 50 kDa BFA-dependent ADP-ribosylation substrate; C-terminal-binding protein 3 (CtBP3); Ctbp3; C-terminal binding protein 1; brefeldin A-ribosylated substrate
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4B6
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CtBP1
Purification	affinity purified by Protein A
Predicted MW.	48 kDa
Observed MW.	45 kDa
Subcellular Location	N/A
Gene ID	1487
Swiss-Prot	Q13363
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CTBP1 monoclonal antibody, unconjugated (SLA1957M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CTBP1 Monoclonal Antibody, Unconjugated(SLA1957M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CTBP1 Monoclonal Antibody, Unconjugated(SLA1957M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CTBP1 Monoclonal Antibody, Unconjugated(SLA1957M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CTBP1 Monoclonal Antibody, Unconjugated(SLA1957M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-CTBP1 antibody (SLA1957M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.