Product Name	Recombinant Rabbit Anti-SGCA Monoclonal Antibody
Cat No.	SLA1575M
Chinese Name	α肌萎缩糖蛋白2重组兔单抗
Alias	50DAG; ADL; DAG2; DMDA2; LGMD2D; LGMDR3; SCARMD1; adhalin; Asg; SGCA_HUMAN; SGCA; Alpha-SG; 50 kDa dystrophin-associated glycoprotein (50DAG); Dystroglycan-2; SGCA_MOUSE; Q5SWB2_MOUSE; SGCA_RABIT; sarcoglycan alpha; sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); 50kD DAG; limb girdle muscular dystrophy 2D
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4A2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human alpha Sarcoglycan
Purification	affinity purified by Protein A
Predicted MW.	43 kDa
Observed MW.	50 kDa
Gene ID	6442
Swiss-Prot	Q16586
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with alpha Sarcoglycan monoclonal antibody, unconjugated (SLA1575M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (SLA1575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (SLA1575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (SLA1575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (SLA1575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (SLA1575M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.