Product Name	Recombinant Rabbit Anti-Drosha Monoclonal Antibody
Cat No.	SLA1562M
Chinese Name	核糖核酸酶3/Drosha重组兔单抗
Alias	ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN; 1110013A17Rik; RNC_HUMAN; DROSHA; Protein Drosha; Ribonuclease III (RNase III); p241; 3.1.26.3; RNC_MOUSE; drosha ribonuclease III; ribonuclease type III, nuclear; drosha, ribonuclease type III; drosha, double-stranded RNA-specific endoribonuclease
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12G5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Drosha
Purification	affinity purified by Protein A
Predicted MW.	159 kDa
Observed MW.	180 kDa
Subcellular Location	Nucleus
Gene ID	29102
Swiss-Prot	Q9NRR4
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Drosha monoclonal antibody, unconjugated (SLA1562M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Drosha Monoclonal Antibody, Unconjugated (SLA1562M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Drosha Monoclonal Antibody, Unconjugated (SLA1562M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Drosha Monoclonal Antibody, Unconjugated (SLA1562M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Drosha Monoclonal Antibody, Unconjugated (SLA1562M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Drosha Monoclonal Antibody, Unconjugated (SLA1562M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.