Product Name	Recombinant Rabbit Anti-ASNS Monoclonal Antibody
Cat No.	SLA1103M
Chinese Name	天冬酰胺合成酶单克隆抗体
Alias	ASNSD; TS11; ASNS_HUMAN; ASNS; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; 6.3.5.4; ASNS_MOUSE; ASNS_RAT; asparagine synthetase (glutamine-hydrolyzing); asparagine synthetase
Host Species	Mouse
Isotype	IgG1, Kappa
Clonality	Monoclonal
Clone No.	2A9
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human ASNS
Purification	affinity purified by Protein A
Predicted MW.	64 kDa
Observed MW.	64 kDa
Subcellular Location	Cytoplasm
Gene ID	440
Swiss-Prot	P08243
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ASNS monoclonal antibody, unconjugated (SLA1103M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (SLA1103M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (SLA1103M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (SLA1103M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (SLA1103M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ASNS Monoclonal Antibody, Unconjugated (SLA1103M) at 1:300 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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