Product Name	Recombinant Rabbit Anti-CHI3L1 Monoclonal Antibody
Cat No.	SLA1048M
Chinese Name	软骨糖蛋白39重组兔单抗
Alias	ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; YK-40; YKL-40; YKL40; YYL-40; hCGP-39; Brp39; Chil1; GP38K; CH3L1_HUMAN; CHI3L1; 39 kDa synovial protein; Cartilage glycoprotein 39 (CGP-39 | GP-39 | hCGP-39); CH3L1_MOUSE; BRP39 protein; Breast regression protein 39; Cartilage glycoprotein 39 (CGP-39 | GP-39); CH3L1_PONAB; CH3L1_PIG; 38 kDa heparin-binding glycoprotein; Signal-processing protein; CH3L1_RAT; chitinase 3 like 1; chitinase 3-like 1 (cartilage glycoprotein-39); cartilage glycoprotein-39;
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1H3
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CHI3L1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	42 kDa
Observed MW.	30-40 kDa
Gene ID	1116
Swiss-Prot	P36222
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. THP-1 (H) treated with PMA (100 ng/mL) for 24 h, Replace with fresh complete culture medium and continue to culture for 24 h, + hIFN-α (500U/mL) for 24 h, then with Brefeldin A (1µg/ml) for 3 h, 25 μg total protein per lane of cell lysates (see on figure) probed with CHI3L1 monoclonal antibody, unconjugated (SLA1048M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (SLA1048M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (SLA1048M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (SLA1048M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (SLA1048M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (SLA1048M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (SLA1048M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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