Product Name	Recombinant Rabbit Anti-BrdU Monoclonal Antibody
Cat No.	SLA0945M
Chinese Name	5-溴脱氧尿嘧啶核苷重组兔单抗
Alias	Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.; BrdU (Proliferation Marker);
Host Species	Rabbit
Isotype	IgG1, k
Clonality	Monoclonal
React Species	Species independent
Applications	IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated BrdU
Purification	affinity purified by Protein A
Concentration	1mg/ml
Subcellular Location	Nucleus
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Jejunum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (SLA0945M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.