DESCRIPTION

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily. CD84 is a singlechain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	714.6	69.0	9.7
2	8	169.9	6.3	3.7
3	8	80.5	3.1	3.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	691.9	60.7	8.8
2	16	169.0	12.7	7.5
3	16	80.4	7.5	9.3

RECOVERY

The recovery of mouse CD84 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:400	84	78-95
	1:800	93	84-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:100.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	115
	Range (%)	108-121
1:8	Average% of Expected	109
	Range (%)	107-112
1:16	Average% of Expected	108
	Range (%)	100-116

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse CD84 One-Step ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)