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Recombinant Rabbit Anti-PCBP2 Monoclonal Antibody
Recombinant Rabbit Anti-PCBP2 Monoclonal Antibody
HNRNPE2; HNRPE2; hnRNP-E2; Hnrpx; alphaCP-2; PCBP2_HUMAN; PCBP2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2 (hnRNP E2); PCBP2_MOUSE; CTBP (CBP); Putative heterogeneous nuclear ribonucleoprotein X (hnRNP X); Cbp; Hnrnpx; poly(rC) binding protein
Total $200.0
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Regular members: $160.0
  • NO.:SLA2628M
    React Species:Human, Mouse, Rat
    Immunogen Species:Rabbit
    Clonality:Recombinant
    Applications:WB, IHC-P, IHC-F, IF, ICC/IF, IP
    Gene name:PCBP2
    UniProt Entry:Q15366
    NCBI Gene Entry:5094
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Product NameRecombinant Rabbit Anti-PCBP2 Monoclonal Antibody
Cat No.SLA2628M
Chinese Name异构核糖核蛋白E2重组兔单抗
AliasHNRNPE2; HNRPE2; hnRNP-E2; Hnrpx; alphaCP-2; PCBP2_HUMAN; PCBP2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2 (hnRNP E2); PCBP2_MOUSE; CTBP (CBP); Putative heterogeneous nuclear ribonucleoprotein X (hnRNP X); Cbp; Hnrnpx; poly(rC) binding protein 2; poly(rC)-binding protein 2; heterogenous nuclear ribonucleoprotein E2
Host SpeciesRabbit
IsotypeIgG
ClonalityMonoclonal
Clone No.14H1
React SpeciesHuman,Mouse,Rat
ApplicationsWB (1:500-2000)
IHC-P (1:100-500)
IHC-F (1:100-500)
IF (1:100-500)
ICC/IF (1:50-200)
IP (1:20-50)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
ImmunogenA synthesized peptide derived from human PCBP2
Purificationaffinity purified by Protein A
Predicted MW.39 kDa
Observed MW.39-42 kDa
Gene ID5094
Swiss-ProtQ15366
Storage0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
BackgroundSingle-stranded nucleic acid binding protein that binds preferentially to oligo dC.Major cellular poly(rC)-binding protein.Acts as a negative regulator of antiviral signaling .
AttentionThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

SLA2628M Figure 1

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PCBP2 monoclonal antibody, unconjugated (SLA2628M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

SLA2628M Figure 2

Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2628M Figure 3

Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2628M Figure 4

Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2628M Figure 5

Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2628M Figure 6

Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2628M Figure 7

Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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