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Product Name Recombinant Rabbit Anti-PCBP2 Monoclonal Antibody Cat No. SLA2628M Chinese Name 异构核糖核蛋白E2重组兔单抗 Alias HNRNPE2; HNRPE2; hnRNP-E2; Hnrpx; alphaCP-2; PCBP2_HUMAN; PCBP2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2 (hnRNP E2); PCBP2_MOUSE; CTBP (CBP); Putative heterogeneous nuclear ribonucleoprotein X (hnRNP X); Cbp; Hnrnpx; poly(rC) binding protein 2; poly(rC)-binding protein 2; heterogenous nuclear ribonucleoprotein E2 Host Species Rabbit Isotype IgG Clonality Monoclonal Clone No. 14H1 React Species Human,Mouse,Rat Applications WB (1:500-2000)
IHC-P (1:100-500)
IHC-F (1:100-500)
IF (1:100-500)
ICC/IF (1:50-200)
IP (1:20-50)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.Immunogen A synthesized peptide derived from human PCBP2 Purification affinity purified by Protein A Predicted MW. 39 kDa Observed MW. 39-42 kDa Gene ID 5094 Swiss-Prot Q15366 Storage 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.Background Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.Major cellular poly(rC)-binding protein.Acts as a negative regulator of antiviral signaling . Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Validation Images
Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PCBP2 monoclonal antibody, unconjugated (SLA2628M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation PCBP2 Monoclonal Antibody, Unconjugated (SLA2628M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.
+86 571 56623320
[email protected]
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