Product Name	Recombinant Rabbit Anti-FADS1 Monoclonal Antibody
Cat No.	SLA2369M
Chinese Name	脂肪酸去饱和酶1重组兔单抗
Alias	D5D; FADS6; FADSD5; LLCDL1; TU12; 0710001O03Rik; A930006B21Rik; DSD; FADS1_HUMAN; FADS1; Delta(5) fatty acid desaturase (D5D | Delta(5) desaturase | Delta-5 desaturase); Fatty acid desaturase 1; 1.14.19.44; FADS1_MOUSE; FADS1_RAT; delta-5 desaturase; acyl-CoA (8-3)-desaturase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	17H18
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human FADS1
Purification	affinity purified by Protein A
Predicted MW.	52 kDa
Observed MW.	52 kDa
Subcellular Location	Cytoplasm
Gene ID	3992
Swiss-Prot	O60427
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FADS1 Monoclonal Antibody, Unconjugated (SLA2369M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FADS1 Monoclonal Antibody, Unconjugated (SLA2369M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FADS1 Monoclonal Antibody, Unconjugated (SLA2369M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FADS1 Monoclonal Antibody, Unconjugated (SLA2369M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FADS1 Monoclonal Antibody, Unconjugated (SLA2369M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The A673 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-FADS1 antibody (SLA2369M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (SL40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 7. 4% Paraformaldehyde-fixed A673 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FADS1) monoclonal Antibody, unconjugated (SLA2369M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.