DESCRIPTION

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity. CD134 can interact with TRAF2, TRAF3, and TRAF5.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,262.5	54.8	4.3
2	8	301.1	7.6	2.5
3	8	148.0	8.7	5.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,313.3	82.1	6.3
2	16	303.2	12.1	4.0
3	16	149.5	11.7	7.8

RECOVERY

The recovery of mouse CD134/OX40 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:8	82	79-84
Cell culture supernatant	1:8	92	76-103
Tissue lysate	1:32	92	88-96

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The tissue lysate was initially diluted 1:2.)

		Mouse serum	Cell culture supernatant	Tissue lysate
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	97	107	98
	Range (%)	95-99	98-116	96-100
1:8	Average% of Expected	99	104	102
	Range (%)	87-111	91-117	99-104
1:16	Average% of Expected	-	105	113
	Range (%)	-	101-109	112-114

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse CD134/OX40 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)