Product Name	Recombinant Rabbit Anti-NCAM1 Monoclonal Antibody
Cat No.	SLA0363M
Chinese Name	小鼠抗人CD56 (NCAM)单克隆抗体
Alias	CD56; MSK39; NCAM; E-NCAM; NCAM-1; N-CAM; N-CAM-1; NCAM-C; NCAMC; NCAM1_HUMAN; NCAM1; NCAM1_MOUSE; NCAM1_RAT; neural cell adhesion molecule 1
Host Species	Mouse
Isotype	Mouse IgG2a, k
Clonality	Monoclonal
Clone No.	6C8
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CD56
Purification	affinity purified by Protein G
Predicted MW.	92 kDa
Observed MW.	140 kDa
Subcellular Location	Membrane
Gene ID	4684
Swiss-Prot	P13591
Storage	0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated(SLA0363M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated(SLA0363M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated(SLA0363M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated(SLA0363M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated(SLA0363M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated(SLA0363M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining
Figure 7. scatter diagram showing peripheral blood lymphocytes stained with CD56. The cells were incubated with the antibody (SLA0363M) for 30 min at 22°C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.