DESCRIPTION

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	9.36	0.72	7.71
2	8	2.21	0.11	4.97
3	8	0.97	0.08	8.32

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	8.69	0.83	9.50
2	16	2.13	0.16	7.38
3	16	1.01	0.08	7.48

RECOVERY

The recovery of human PSMD1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:128	111	102-121
Tissue lysate	1:32	93	70-111

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:8. The tissue lysate was initially diluted 1:16. )

		Cell lysate	Tissue lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	106	100
	Range (%)	105-106	99-102
1:8	Average% of Expected	109	115
	Range (%)	108-110	108-123
1:16	Average% of Expected	104	112
	Range (%)	101-107	111-113

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human PSMD1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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