DESCRIPTION

PSMC3IP (PSMC3-interacting protein), also known as TBPIP, GT198, HUMGT198A, or Hop2, is a nuclear protein that plays important roles in meiotic recombination, DNA repair, transcriptional regulation, cancer development, and cell cycle regulation. PSMC3IP is a subunit of the PSMC3IP/MND1 complex, interacting with PSMC3/TBP1 to stimulate DMC1and RAD51-mediated strand exchange during meiosis. Additionally, PSMC3IP acts as a coactivator of ligand-dependent transcription, participating in transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in the PSMC3IP gene can lead to XX female gonadal dysgenesis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,223.7	62.9	5.1
2	8	302.9	7.9	2.6
3	8	101.5	3.5	3.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,217.2	68.3	5.6
2	16	314.7	14.6	4.7
3	16	102.2	5.0	4.9

RECOVERY

The recovery of human PSMC3IP spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:640	87	82-91
	1:1,280	89	89-90

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	110
	Range (%)	92-127
1:8	Average% of Expected	85
	Range (%)	83-87
1:16	Average% of Expected	98
	Range (%)	93-102

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PSMC3IP Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)