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Rabbit Anti-UNG Polyclonal Antibody
Rabbit Anti-UNG Polyclonal Antibody
UNG_HUMAN; UNG; UDG; 3.2.2.27; DGU; UNG1; UNG15; HIGM4; HIGM5; UNG2; uracil DNA glycosylase; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2;
Total $295.0
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Regular members: $236.0
  • NO.:SLA0300
    concentration:1mg/ml
    React Species:Human, Mouse, Rat
    Immunogen Species:Rabbit
    Clonality:Polyclonal
    Applications:WB, IHC-P, IHC-F, IF, ELISA
    Gene name:UNG
    UniProt Entry:P13051
    NCBI Gene Entry:7374
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Details

 

Product NameRabbit Anti-UNG Polyclonal Antibody
Cat No.SLA0300
Chinese Name尿嘧啶-DNA糖基化酶抗体
AliasUNG_HUMAN; UNG; UDG; 3.2.2.27; DGU; UNG1; UNG15; HIGM4; HIGM5; UNG2; uracil DNA glycosylase; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2;
Host SpeciesRabbit
IsotypeIgG
ClonalityPolyclonal
React SpeciesHuman,Mouse,Rat
ApplicationsWB (1:500-2000)
IHC-P (1:100-500)
IHC-F (1:100-500)
IF (1:100-500)
ELISA (1:5000-10000)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
ImmunogenKLH conjugated synthetic peptide derived from human UNG
Purificationaffinity purified by Protein A
Concentration1mg/ml
Predicted MW.35 kDa
Observed MW.38 kDa
Gene ID7374
Swiss-ProtP13051
Storage0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
AttentionThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

SLA0300 Figure 1

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with UDG polyclonal antibody, unconjugated (SLA0300) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

SLA0300 Figure 2

Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA0300 Figure 3

Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA0300 Figure 4

Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA0300 Figure 5

Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA0300 Figure 6

Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA0300 Figure 7

Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA0300 Figure 8

Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UDG Polyclonal Antibody, Unconjugated (SLA0300) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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