Product Name	Recombinant Rabbit Anti-MCM6 Monoclonal Antibody
Cat No.	SLA2669M
Chinese Name	微小染色体维持缺陷蛋白6重组兔单抗
Alias	MCG40308; Mis5; P105MCM; ASP-l1; D1Wsu22e; Mcmd6; MCM6_BOVIN; MCM6; 3.6.4.12; MCM6_HUMAN; MCM6_MOUSE; Mis5 homolog; MCM6_RAT; Intestinal DNA replication protein; minichromosome maintenance complex component 6; minichromosome maintenance deficient (mis5, S. pombe) 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae); minichromosome maintenance deficient 6 homolog (S. cerevisiae); MIS5 homolog (S.pombe)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3C11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human MCM6
Purification	affinity purified by Protein A
Predicted MW.	93 kDa
Observed MW.	98 kDa
Subcellular Location	Nucleus
Gene ID	4175
Swiss-Prot	Q14566
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MCM6) monoclonal Antibody, unconjugated (SLA2669M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min. DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with MCM6 monoclonal antibody, unconjugated (SLA2669M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MCM6 Monoclonal Antibody, Unconjugated (SLA2669M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MCM6 Monoclonal Antibody, Unconjugated (SLA2669M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MCM6 Monoclonal Antibody, Unconjugated (SLA2669M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MCM6 Monoclonal Antibody, Unconjugated (SLA2669M) at 1:1000 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MCM6 antibody (SLA2669M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed	Figure 8. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MCM6) monoclonal Antibody, unconjugated (SLA2669M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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