Product Name	Recombinant Rabbit Anti-c-Jun&JunD (p-S73/S100) Monoclonal Antibody
Cat No.	SLA2657M
Chinese Name	磷酸化原癌基因c-Jun/JunD重组兔单抗
Alias	AP-1; AP1; c-Jun; cJUN; p39; Junc; JUN_HUMAN; JUN; Activator protein 1 (AP1); Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; JUN_MOUSE; AH119; V-jun avian sarcoma virus 17 oncogene homolog (Jun A); JUN_PIG; JUN_RAT; Rjg-9; Jun proto-oncogene, AP-1 transcription factor subunit; jun oncogene; c-Jun & JunD (phospho-S73/S100); p-c-Jun & JunD; phospho-c-Jun & JunD; c-Jun & JunD (phospho-Ser73/Ser100)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6D2
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human c Jun around the phosphorylation site of S73
Purification	affinity purified by Protein A
Predicted MW.	36,35 kDa
Observed MW.	44 kDa
Subcellular Location	Nucleus
Gene ID	3725
Swiss-Prot	P05412
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. NIH/3T3 (M) cells were treated with or without anisomycin (25μg/mL) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-c-Jun (Thr91+Thr93) monoclonal antibody, unconjugated (SLA2657M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated(SLA2657M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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