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Recombinant Rabbit Anti-IFT88 Monoclonal Antibody
Recombinant Rabbit Anti-IFT88 Monoclonal Antibody
IFT88_HUMAN; IFT88; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10 (TPR repeat protein 10); TG737; TTC10; D13S1056E; DAF19; hTg737; intraflagellar transport 88; tetratricopeptide repeat domain 10; intraflage
Total $200.0
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  • NO.:SLA2605M
    React Species:Human, Mouse, Rat
    Immunogen Species:Rabbit
    Clonality:Recombinant
    Applications:WB, IHC-P, IHC-F, IF
    Gene name:IFT88
    UniProt Entry:Q13099
    NCBI Gene Entry:8100
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Product NameRecombinant Rabbit Anti-IFT88 Monoclonal Antibody
Cat No.SLA2605M
Chinese NameIFT88重组兔单抗
AliasIFT88_HUMAN; IFT88; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10 (TPR repeat protein 10); TG737; TTC10; D13S1056E; DAF19; hTg737; intraflagellar transport 88; tetratricopeptide repeat domain 10; intraflagellar transport 88 homolog (Chlamydomonas); polaris homolog
Host SpeciesRabbit
IsotypeIgG
ClonalityMonoclonal
Clone No.4A12
React SpeciesHuman,Mouse,Rat
ApplicationsWB (1:500-2000)
IHC-P (1:100-500)
IHC-F (1:100-500)
IF (1:100-500)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
ImmunogenA synthesized peptide derived from human IFT88
Purificationaffinity purified by Protein A
Predicted MW.93 kDa
Observed MW.80 kDa
Gene ID8100
Swiss-ProtQ13099
Storage0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
BackgroundInvolved in primary cilium biogenesis.
AttentionThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

SLA2605M Figure 1

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with IFT88 monoclonal antibody, unconjugated (SLA2605M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

SLA2605M Figure 2

Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 3

Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 4

Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 5

Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 6

Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 7

Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 8

Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA2605M Figure 9

Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IFT88 Monoclonal Antibody, Unconjugated(SLA2605M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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  • Minimum order: 1 unit
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