Product Name	Recombinant Rabbit Anti-PAH Monoclonal Antibody
Cat No.	SLA2584M
Chinese Name	苯丙氨酸羟化酶4重组兔单抗
Alias	PH; PKU; PKU1; PH4H_HUMAN; PAH; Phe-4-monooxygenase; 1.14.16.1; PH4H_MOUSE; PH4H_RAT; phenylalanine hydroxylase; L-phenylalanine hydroxylase; phenylalanine-4-hydroxylase; phenylalanine 4-monooxygenase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4H12
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PAH
Purification	affinity purified by Protein A
Predicted MW.	52 kDa
Observed MW.	51 kDa
Subcellular Location	Cytoplasm
Gene ID	5053
Swiss-Prot	P00439
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PAH monoclonal antibody, unconjugated (SLA2584M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Fetal liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (SLA2584M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.