Product Name	Recombinant Rabbit Anti-CHMP3 Monoclonal Antibody
Cat No.	SLA2159M
Chinese Name	液泡蛋白分选蛋白24重组兔单抗
Alias	VPS24; CGI-149; NEDF; 25.1; 4921505F14Rik; 9130011K15Rik; CGI-49; D6Ertd286e; fb47g01; wu:fb47g01; zgc:76972; CHMP3_BOVIN; CHMP3; Chromatin-modifying protein 3; Vacuolar protein sorting-associated protein 24; CHMP3_HUMAN; Neuroendocrine differentiation factor; Vacuolar protein sorting-associated protein 24 (hVps24); CGI149; CHMP3_MOUSE; CHMP3_RAT; Vacuolar protein sorting-associated protein 24 (rVps24p); CHMP3_DANRE; Vacuolar protein-sorting-associated protein 24; charged multivesicular body protein 3; vacuolar protein sorting 24 (yeast); vacuolar protein sorting 24 homolog (S. cerevisiae)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1H2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human VPS24
Purification	affinity purified by Protein A
Predicted MW.	25 kDa
Observed MW.	28 kDa
Subcellular Location	N/A
Gene ID	51652
Swiss-Prot	Q9Y3E7
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with VPS24 monoclonal antibody, unconjugated (SLA2159M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VPS24 Monoclonal Antibody, Unconjugated (SLA2159M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-VPS24 antibody (SLA2159M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.