Product Name	Recombinant Rabbit Anti-RBM8A Monoclonal Antibody
Cat No.	SLA2108M
Chinese Name	RBM8A重组兔单抗
Alias	RBM8A_HUMAN; RBM8A; Binder of OVCA1-1 (BOV-1); RNA-binding motif protein 8A; RNA-binding protein Y14; Ribonucleoprotein RBM8A; RBM8; BOV-1A; BOV-1B; BOV-1C; C1DELq21.1; DEL1q21.1; MDS014; RBM8B; TAR; Y14; ZNRP; ZRNP1; RNA binding motif protein 8A; binder of OVCA1; exon junction complex core component Y14
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	12B4
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human RBM8A
Purification	affinity purified by Protein A
Predicted MW.	20 kDa
Observed MW.	21 kDa
Subcellular Location	N/A
Gene ID	9939
Swiss-Prot	Q9Y5S9
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Required for pre-mRNA splicing as component of the spliceosome.Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RBM8A) monoclonal Antibody, unconjugated (SLA2108M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min. DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with RBM8A monoclonal antibody, unconjugated (SLA2108M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBM8A Monoclonal Antibody, Unconjugated (SLA2108M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBM8A Monoclonal Antibody, Unconjugated (SLA2108M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBM8A Monoclonal Antibody, Unconjugated (SLA2108M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBM8A Monoclonal Antibody, Unconjugated (SLA2108M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBM8A Monoclonal Antibody, Unconjugated (SLA2108M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-RBM8A antibody (SLA2108M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 9. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RBM8A) monoclonal Antibody, unconjugated (SLA2108M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.