Product Name	Recombinant Rabbit Anti-H2AC4 Monoclonal Antibody
Cat No.	SLA2951M
Chinese Name	组蛋白H2A重组兔单抗
Alias	H2A.1; H2A.2; H2A/a; H2AC4; H2AFA; HIST1H2AE; H2AB; HIST2H2AB; H2A/m; H2AC8; H2AFM; HIST1H2AB; H2A; H2A-GL101; H2A/q; H2AFQ; HIST2H2AC; 1190022L06; H2A1B_HUMAN; Histone H2A.2; Histone H2A/a; Histone H2A/m; H2A1_HUMAN; H2AC11; H2AC13; H2AC15; H2AC16; H2AC17; Histone H2A/ptl; H2AFP; HIST1H2AG; H2AFC; HIST1H2AI; H2AFD; HIST1H2AK; H2AFI; HIST1H2AL; H2AFN; HIST1H2AM; H2A1D_HUMAN; H2AC7; Histone H2A.3; Histone H2A/g; H2AFG; HIST1H2AD; H2A2C_HUMAN; H2AC20; H2A-clustered histone 20; Histone H2A-GL101; Histone H2A/q; H2A2A_HUMAN; H2AC18; H2AC19; H2A-clustered histone 18; H2A-clustered histone 19; Histone H2A/o; H2AFO; HIST2H2AA; HIST2H2AA3; HIST2H2AA4; H2A3_HUMAN; H2AC25; H2A-clustered histone 25; H2AW; HIST3H2A; H2A2B_HUMAN; H2AC21; H2A-clustered histone 21; H2A1C_HUMAN; H2AC6; H2A-clustered histone 6; Histone H2A/l; H2AFL; HIST1H2AC; H2A1H_HUMAN; H2AC12; H2A-clustered histone 12; Histone H2A/s; HIST1H2AH; H2A1A_HUMAN; H2AC1; H2A-clustered histone 1; Histone H2A/r; H2AFR; HIST1H2AA; H2A1J_HUMAN; H2AC14; Histone H2A/e; H2AFE; HIST1H2AJ; H2A4_MOUSE; H2A1_MOUSE; H2A clustered histone 4; H2A histone family, member M; histone 1, H2ab; histone cluster 1, H2ab; histone cluster 1 H2A family member b
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2F12
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Histone H2A
Purification	affinity purified by Protein A
Predicted MW.	14 kDa
Observed MW.	14 kDa
Gene ID	3012
Swiss-Prot	P04908
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Histone H2A monoclonal antibody, unconjugated (SLA2951M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H2A Monoclonal Antibody, Unconjugated (SLA2951M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.