Product overview

Sunlong Medical™ Cetuximab ELISA Kit (Catalog No. ADA0022) is an enzyme-linked immunosorbent assay kit for detection of the target in serum, plasma, cell culture supernatant.

Assay principle

OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human EGFR antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Cetuximab in the sample competitively binds to the pre-coated protein with biotin-labeled Cetuximab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Cetuximab bound in the initial step. The color development is stopped and the intensity of the color is measured. OTHER SUPPLIES REQUIRED • Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm or 620 nm. • Pipettes and pipette tips. • Deionized or distilled water. • 500 mL graduated cylinder. • Squirt bottle, manifold dispenser, or automated microplate washer. • Test tubes for dilution of standards. SAMPLE COLLECTION & STORAGE The sample colle

Specifications

• See the instruction manual (PDF) for detailed specifications.

Sample type

serum, plasma, cell culture supernatant

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)