Product Name	Recombinant Rabbit Anti-HuC&HuD Monoclonal Antibody
Cat No.	SLA3412M
Alias	ELAV-like protein 3/4;Hu-antigen D,HuD,Paraneoplastic encephalomyelitis antigen HuD,ELAV-like protein 3,Hu-antigen C,HuC,Paraneoplastic cerebellar degeneration-associated antigen,Paraneoplastic limbic encephalitis antigen 21,
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6B8
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ELISA (1:5000-10000) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Predicted MW.	40,42 kDa
Observed MW.	40-42 kDa
Gene ID	1995
Swiss-Prot	Q14576
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with HuC&HuD monoclonal antibody, unconjugated (SLA3412M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HuC&HuD Monoclonal Antibody, Unconjugated (SLA3412M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.