Product Name	Recombinant Rabbit Anti-ERp29 Monoclonal Antibody
Cat No.	SLA1900M
Chinese Name	内质网蛋白29重组兔单抗
Alias	C12orf8; ERp28; ERp31; HEL-S-107; PDI-DB; PDIA9; 1200015M03Rik; 2810446M09Rik; ERP29_BOVIN; ERP29; ERP29_HUMAN; Endoplasmic reticulum resident protein 28 (ERp28); Endoplasmic reticulum resident protein 31 (ERp31); ERP29_MOUSE; ERP29_RAT; endoplasmic reticulum protein 29; chromosome 12 open reading frame 8; protein disulfide isomerase family A, member 9
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5C11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ERp29
Purification	affinity purified by Protein A
Predicted MW.	29 kDa
Observed MW.	30 kDa
Subcellular Location	N/A
Gene ID	10961
Swiss-Prot	P30040
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ERp29 monoclonal antibody, unconjugated (SLA1900M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp29 Monoclonal Antibody, Unconjugated (SLA1900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 11. The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ERp29 antibody (SLA1900M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.