DESCRIPTION

TXN2 (Thioredoxin2) is also named as TRX2, MTRX, Mt-TRX and regulates the mitochondrial redox state and plays an important role in cell proliferation. In contrast to cytosolic TRX1, TRX2 is synthesized with an N-terminal mitochondrial targeting sequence that drives its exclusive localization to the mitochondrial matrix. It has been shown that cells conditionally deficient in TRX2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. Within mitochondria, TRX2 participates in the thioredoxin/peroxiredoxin antioxidant axis, which operates in parallel with the glutathione system to maintain the organelle's redox balance and ATP-generating capacity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	88.90	7.75	8.72
2	8	21.49	1.49	6.95
3	8	12.75	0.56	4.38

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	85.85	7.10	8.27
2	16	22.46	2.12	9.43
3	16	12.53	0.79	6.33

RECOVERY

The recovery of human TRX2/TXN2 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:4	97	93-99
	1:8	99	97-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	101
	Range (%)	92-110
1:8	Average% of Expected	112
	Range (%)	104-120
1:16	Average% of Expected	123
	Range (%)	114-131

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TRX2/TXN2 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)