Product Name	Recombinant Rabbit Anti-PRDX3 Monoclonal Antibody
Cat No.	SLA1639M
Chinese Name	硫氧还蛋白还原酶3重组兔单抗
Alias	MTAP; AOP-1; AOP1; HBC189; MER5; PPPCD; PRO1748; SCAR32; SP-22; prx-III; D0Tohi1; Ef2l; Prx3; SP22; TDXM; PRDX3_BOVIN; PRDX3; Antioxidant protein 1 (AOP-1); Peroxiredoxin-3; Protein SP-22; Thioredoxin-dependent peroxiredoxin 3; 1.11.1.24; PRDX3_HUMAN; Peroxiredoxin III (Prx-III); Protein MER5 homolog; PRDX3_MOUSE; PRX III; Perioredoxin-3; Protein MER5; PRDX3_RAT; Peroxiredoxin-3 (PRX-3); peroxiredoxin 3; antioxidant protein 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	17A17
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PRDX3
Purification	affinity purified by Protein A
Predicted MW.	28 kDa
Observed MW.	20 kDa
Subcellular Location	N/A
Gene ID	10935
Swiss-Prot	P30048
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Peroxiredoxin 3) monoclonal Antibody, unconjugated (SLA1639M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with Peroxiredoxin 3 monoclonal antibody, unconjugated (SLA1639M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated(SLA1639M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated(SLA1639M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated(SLA1639M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated(SLA1639M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated(SLA1639M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated (SLA1639M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin 3 Monoclonal Antibody, Unconjugated (SLA1639M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei	Figure 10. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Peroxiredoxin 3 antibody (SLA1639M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 11. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Peroxiredoxin 3) monoclonal Antibody, unconjugated (SLA1639M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, SL40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.