Product Name	Recombinant Rabbit Anti-PFKM Monoclonal Antibody
Cat No.	SLA3025M
Chinese Name	肌肉型6磷酸果糖激酶重组兔单抗
Alias	ATP-PFK; PFK-M; M-PFK; PFK-A; GSD7; PFK-1; PFK1; PFKA; PFKX; PPP1R122; Pfk-4; Pfk4; PFMK; PFK; PFKAM_BOVIN; PFKM; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A (PFK-A); Phosphohexokinase; 2.7.1.11; PFKAM_CANLF; PFKAM_HORSE; PFKAM_HUMAN; PFKAM_MOUSE; PFKAM_PIG; PFKAM_RABIT; PFKAM_RAT; phosphofructokinase, muscle; phosphofructokinase, polypeptide X; protein phosphatase 1, regulatory subunit 122
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2F5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PFK M
Purification	affinity purified by Protein A
Predicted MW.	85 kDa
Observed MW.	82 kDa
Subcellular Location	N/A
Gene ID	5213
Swiss-Prot	P08237
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PFKM monoclonal antibody, unconjugated (SLA3025M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFKM Monoclonal Antibody, Unconjugated (SLA3025M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFKM Monoclonal Antibody, Unconjugated (SLA3025M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFKM Monoclonal Antibody, Unconjugated (SLA3025M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFKM Monoclonal Antibody, Unconjugated (SLA3025M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFKM Monoclonal Antibody, Unconjugated (SLA3025M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFKM Monoclonal Antibody, Unconjugated (SLA3025M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PFKM antibody (SLA3025M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.