Product Name	Recombinant Rabbit Anti-MYH10 Monoclonal Antibody
Cat No.	SLA3292M
Alias	NMMHC-IIB; NMMHCB; 5730504C04Rik; 9330167F11Rik; Fltn; Myhn-2; Myhn2; NM2B; NMHC II-B; NMHC-B; NMHCII-B; NMMHC II-b; NMMHC-B; SMemb; mKIAA3005; MCH-B; MYH10_BOVIN; MYH10; Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; Non-muscle myosin heavy chain B (NMMHC-B); Non-muscle myosin heavy chain IIb (NMMHC II-b | NMMHC-IIB); MYH10_HUMAN; Q5SV64_MOUSE; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; MYH10_MOUSE; Q8BXF2_MOUSE; MYH10_RAT; myosin, heavy polypeptide 10, non-muscle; Myosin-10 (Cellular myosin heavy chain, type B) (Myosin heavy chain 10) (Myosin heavy chain, non-muscle IIb) (Non-muscle myosin heavy chain B) (NMMHC-B) (Non-muscle myosin heavy chain IIb) (NMMHC II-b) (NMMHC-IIB)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2G2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ELISA (1:5000-10000) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Gene ID	4628
Swiss-Prot	P35580
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYH10 | NMMHC-IIB Monoclonal Antibody, Unconjugated (SLA3292M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.