Product Name	Recombinant Rabbit Anti-SRSF1 Monoclonal Antibody
Cat No.	SLA1718M
Chinese Name	选择性剪接因子1重组兔单抗
Alias	ASF; NEDFBA; SF2; SF2p33; SFRS1; SRp30a; 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; SRSF1_HUMAN; SRSF1; Alternative-splicing factor 1 (ASF-1); Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit; SRSF1_MOUSE; ASF/SF2; Pre-mRNA-splicing factor SRp30a; serine and arginine rich splicing factor 1; serine/arginine-rich splicing factor 1; splicing factor 2; alternate splicing factor; SR splicing factor 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4A12
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human SRSF1
Purification	affinity purified by Protein A
Predicted MW.	28 kDa
Observed MW.	30 kDa
Subcellular Location	N/A
Gene ID	6426
Swiss-Prot	Q07955
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SRSF1/SF2 monoclonal antibody, unconjugated (SLA1718M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 10. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SRSF1/SF2 Monoclonal Antibody, Unconjugated (SLA1718M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 13. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SRSF1/SF2 antibody (SLA1718M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.