Product Name	Recombinant Rabbit Anti-CD63 Monoclonal Antibody
Cat No.	SLA0380M
Chinese Name	人CD63单克隆抗体
Alias	AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40; TSPAN30; CD63_HUMAN; CD63; Granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Lysosome integral membrane protein 1 (Limp1); Melanoma-associated antigen ME491; Ocular melanoma-associated antigen; Tetraspanin-30 (Tspan-30); CD63 molecule; CD63 antigen (melanoma 1 antigen); AD1 antigen
Host Species	Mouse
Isotype	Mouse IgG1, k
Clonality	Monoclonal
Clone No.	3B12
React Species	Human
Applications	Flow-Cyt (1μg/Test) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen
Purification	affinity purified by Protein G
Predicted MW.	26 kDa
Observed MW.	30-50 kDa
Subcellular Location	Cytoplasm
Gene ID
Swiss-Prot
Storage	0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. The A375 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-Human CD63 antibody (SLA0380M): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG (SL0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed Figure 2. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (human CD63) monoclonal Antibody, unconjugated (SLA0380M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.