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Recombinant Rabbit Anti-TEAD1 Monoclonal Antibody
Recombinant Rabbit Anti-TEAD1 Monoclonal Antibody
AA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEF-1; TEA domain transcription factor 1; atrophia areata, peripapillary chorioretinal degeneration; TEA domain family member 1 (SV40 transcriptional enhancer factor); transcriptional enhancer factor 1
Total $200.0
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Regular members: $160.0
  • NO.:SLA3059M
    React Species:Human, Mouse, Rat
    Immunogen Species:Rabbit
    Clonality:Recombinant
    Applications:WB, IHC-P, IHC-F, IF, IP
    Gene name:TEAD1
    UniProt Entry:P28347,Q15561,Q15562,Q99594
    NCBI Gene Entry:7003
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Product NameRecombinant Rabbit Anti-TEAD1 Monoclonal Antibody
Cat No.SLA3059M
Chinese NameTEAD1重组兔单抗
AliasAA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEF-1; TEA domain transcription factor 1; atrophia areata, peripapillary chorioretinal degeneration; TEA domain family member 1 (SV40 transcriptional enhancer factor); transcriptional enhancer factor 1
Host SpeciesRabbit
IsotypeIgG
ClonalityMonoclonal
Clone No.4C6
React SpeciesHuman,Mouse,Rat
ApplicationsWB (1:500-2000)
IHC-P (1:100-500)
IHC-F (1:100-500)
IF (1:100-500)
IP (1:20-50)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
ImmunogenA synthesized peptide derived from human TEAD1
Purificationaffinity purified by Protein A
Predicted MW.48 kDa
Observed MW.48-52 kDa
Gene ID7003
Swiss-ProtP28347,Q15561,Q15562,Q99594
Storage0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
BackgroundTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
AttentionThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

SLA3059M Figure 1

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with TEAD1 monoclonal antibody, unconjugated (SLA3059M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

SLA3059M Figure 2

Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 3

Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 4

Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 5

Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 6

Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 7

Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 8

Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 9

Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 10

Figure 10. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 11

Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 12

Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 13

Figure 13. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA3059M Figure 14

Figure 14. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TEAD1 Monoclonal Antibody, Unconjugated (SLA3059M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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