Product Name	Recombinant Rabbit Anti-H2BC21 Monoclonal Antibody
Cat No.	SLA2276M
Chinese Name	甲酰化组蛋白H2B type 2E重组兔单抗
Alias	H2B2E_HUMAN; H2BC21; H2B-clustered histone 21; Histone H2B-GL105; Histone H2B.q (H2B/q); H2BFQ; HIST2H2BE; GL105; H2B; H2B-GL105; H2B.1; H2BE; H2BGL105; H2BQ; H2B clustered histone 21; H2B histone family, member Q; histone 2, H2be; histone cluster 2, H2be; histone cluster 2 H2B family member e; H2BC21 | Histone H2B type 2E (formyl-K109); H2BEK109fo; for-Histone H2B type 2E; formyl-Histone H2B type 2E; for-H2B type 2E; H2BC21 | Histone H2B type 2E (formyl-Lys109)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10D3
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Histone H2B type 2E around the formylation site of K109
Purification	affinity purified by Protein A
Predicted MW.	14 kDa
Observed MW.	14 kDa
Subcellular Location	N/A
Gene ID	8349
Swiss-Prot	Q16778
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Formyl-Histone H2B type 2E (Lys109) monoclonal antibody, unconjugated (SLA2276M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 11. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 13. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Formyl-Histone H2B type 2E (Lys109) Monoclonal Antibody, Unconjugated (SLA2276M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 14. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Formyl-Histone H2B type 2E (Lys109) antibody (SLA2276M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.