Product Name	Recombinant Rabbit Anti-mTOR Monoclonal Antibody
Cat No.	SLA1099M
Chinese Name	雷帕霉素靶蛋白重组小鼠单抗
Alias	FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; 2610315D21Rik; flat; MTOR_HUMAN; MTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin (mTOR); Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; Tyrosine-protein kinase mTOR; 2.7.11.1; MTOR_MOUSE; Rapamycin target protein 1 (RAPT1); MTOR_RAT; mechanistic target of rapamycin kinase; FK506 binding protein 12-rapamycin associated protein 1; mechanistic target of rapamycin (serine/threonine kinase); FK506 binding protein 12-rapamycin associated protein 2; rapamycin target protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; dJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1); mammalian target of rapamycin
Host Species	Mouse
Isotype	IgG1, Kappa
Clonality	Monoclonal
Clone No.	7C11
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human mTOR
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	289 kDa
Observed MW.	289 kDa
Subcellular Location	Cytoplasm
Gene ID	2475
Swiss-Prot	P42345
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 289-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with mTOR monoclonal antibody, unconjugated (SLA1099M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.