Product Name	Recombinant Rabbit Anti-Cd80 Monoclonal Antibody
Cat No.	SLA0503M
Chinese Name	刺激分子B7-1蛋白单克隆抗体
Alias	B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80_HUMAN; CD80; Activation B7-1 antigen; CTLA-4 counter-receptor B7.1 (B7); CD80_MOUSE; Activation B7-1 antigen (B7); CD80 molecule; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B-lymphocyte activation antigen B7
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	11B12
React Species	Human,Mouse
Applications	WB (1:500-2000) Flow-Cyt (1ug/Test) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse CD80 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	32 kDa
Observed MW.	47 kDa
Subcellular Location	Membrane
Gene ID	12519
Swiss-Prot	Q00609
Storage	Size : 50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant mouse CD80 protein, N-GST & C-His(SL41304P) Primary: Anti-CD80 (SLA0503M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 47 kDa Figure 2. Blank control:Raji. Primary Antibody (green line): Mouse Anti-CD80 antibody (SLA0503M) Dilution: 1ug/Test; Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC Dilution: 0.5ug/Test. Isotype control (orange line) : Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.