Product Name	Rabbit Anti-POLK Polyclonal Antibody
Cat No.	SLA0077
Chinese Name	DNA聚合酶κ/DNA pol κ抗体
Alias	DINB1; DINP; POLQ; DNA polymerase kappa; polymerase (DNA directed) kappa; polymerase (DNA) kappa; DINB protein
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human POLK
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	99 kDa
Observed MW.	100 kDa
Subcellular Location	Nucleus
Gene ID	51426
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Prostate tissue lysates Lane 3: Mouse Cerebrum tissue lysates Lane 4: Rat Testis tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Human HeLa cell lysates Lane 7: Human Jurkat cell lysates Lane 8: Human MOLT4 cell lysates Primary: Anti-POLK (SLA0077) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 99 kDa Observed band size: 100 kDa

Figure 2. Measured by its binding ability in a indirect ELISA. Immobilized Human POLK protein, His Tag (Cat. SL25393P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human POLK Antibody, the EC50 is 199.8 ng/mL.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.