Product Name	Recombinant Rabbit Anti-CASP1 Monoclonal Antibody
Cat No.	SLA0842M
Chinese Name	天冬氨酸-胱氨酸特异性蛋白酶家族1 重组兔单抗
Alias	ICE; IL1BC; P45; CASP1_HUMAN; CASP1; CASP-1; Interleukin-1 beta convertase (IL-1BC); Interleukin-1 beta-converting enzyme (ICE | IL-1 beta-converting enzyme); 3.4.22.36; IL1BCE; CASP1_MOUSE; CASP1_RAT; caspase 1; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine peptidase; caspase-1; interleukin 1, beta, convertase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5F17
React Species	Human,Mouse
Applications	WB (1:1000-5000) Flow-Cyt (1μg/Test) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Caspase 1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	46 kDa
Observed MW.	45-47 kDa
Subcellular Location	Cytoplasm
Gene ID	834
Swiss-Prot	P29466
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Caspase-1 monoclonal antibody, unconjugated (SLA0842M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min Figure 2. The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Caspase-1 antibody (SLA0842M): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.