DESCRIPTION

MMP-8 (Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells (PMID:15367487). The high-serum MMP-8 levels (>100 ng/mL) had poor cancer-specific survival (PMID: 29808017). MMP8 also has prognosis in patients with gastric cancer (PMID: 30192205).

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,282.7	23.8	1.9
2	8	322.9	3.5	1.1
3	8	161.9	3.5	2.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,314.0	40.3	3.1
2	16	324.5	6.5	2.0
3	16	166.4	6.2	3.7

RECOVERY

The recovery of human MMP-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:8	106	99-111
	1:16	117	112-121
Saliva	1:32	106	100-111
	1:64	110	104-115
Tissue lysate	1:64	94	88-101
	1:128	102	94-115

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:2. The saliva was initially diluted 1:4. The tissue lysate was initially diluted 1:8.)

		Human plasma	Saliva	Tissue lysate
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	107	100	103
	Range (%)	106-108	98-101	98-108
1:8	Average% of Expected	115	97	109
	Range (%)	108-123	95-98	99-119
1:16	Average% of Expected	107	94	115
	Range (%)	97-116	92-96	103-127

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human MMP-8 produced at Sunlong Medical™.

Ordering & shipping

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