Product Name	Recombinant Rabbit Anti-Ly6g Monoclonal Antibody
Cat No.	SLA0726M
Chinese Name	淋巴细胞抗原6G单克隆抗体
Alias	Gr-1; Gr1; Ly-6G; LY6G_MOUSE; Ly6g; Ly-6G.1;
Host Species	Rat
Isotype	IgG2b
Clonality	Monoclonal
Clone No.	7C7
React Species	Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	14 kDa
Observed MW.	25-40 kDa
Subcellular Location	Membrane
Gene ID	546644
Swiss-Prot	P35461
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Ly6g | Ly-6G monoclonal antibody, unconjugated (SLA0726M) at 1:1000 dilution and 4°C overnight incubation. Followed by HRP conjugated (SL0293G-HRP) at 1:20000 dilution and incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ly-6G Monoclonal Antibody, Unconjugated (SLA0726M) at 1:200 overnight at 4°C, followed by conjugation to SL0293G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ly-6G Monoclonal Antibody, Unconjugated (SLA0726M) at 1:200 overnight at 4°C, followed by conjugation to SL0293G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

• Minimum order: 1 unit
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• Price: USD, per unit (50ul / 100ul / 200ul)