Product Name	Recombinant Rabbit Anti-SELE Monoclonal Antibody
Cat No.	SLA0605M
Chinese Name	E选择素单克隆抗体
Alias	CD62E; ELAM; ELAM1; ESEL; LECAM2; selectin-e; E-selectin; ELAM-1; LYAM2_HUMAN; SELE; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1 (ELAM-1); Leukocyte-endothelial cell adhesion molecule 2 (LECAM2); LYAM2_MOUSE; LYAM2_RAT; selectin E; endothelial adhesion molecule 1
Host Species	Mouse
Clonality	Monoclonal
Clone No.	1G1
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human E-Selectin protein
Purification	affinity purified by Protein A
Predicted MW.	65 kDa
Observed MW.	61 kDa
Subcellular Location	Membrane
Gene ID	6401
Swiss-Prot	P16581
Storage	0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 100 ng rhE-Selectin-His protein (SL43646P) per lane probed with E-Selectin monoclonal antibody respectively, unconjugated (SLA0605M) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(SLA0605M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(SLA0605M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.