Product Name	Recombinant Rabbit Anti-CHAT Monoclonal Antibody
Cat No.	SLA1122M
Chinese Name	ChAT胆碱乙酰转移酶单克隆抗体
Alias	CHOACTASE; CMS1A; CMS1A2; CMS6; B230380D24Rik; CLAT_HUMAN; CHAT; Choline acetylase; 2.3.1.6; CLAT_MOUSE; CLAT_RAT; choline O-acetyltransferase; choline acetyltransferase
Host Species	Mouse
Isotype	IgG1, Kappa
Clonality	Monoclonal
Clone No.	8B6
React Species	Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from mouse ChAT
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	82 kDa
Observed MW.	68 kDa
Subcellular Location	Nucleus
Gene ID	12647
Swiss-Prot	Q03059
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CHAT monoclonal antibody, unconjugated (SLA1122M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHAT Monoclonal Antibody, Unconjugated(SLA1122M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHAT Monoclonal Antibody, Unconjugated(SLA1122M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.