Product Name	MUC13 | DRCC1 Rabbit pAb
Cat No.	SL5877R
Chinese Name	粘蛋白13抗体
Alias	DRCC1; MUC-13; MUC13_HUMAN; MUC13; Down-regulated in colon cancer 1; RECC; mucin 13, cell surface associated; mucin 13, epithelial transmembrane
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human MUC13
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	54 kDa
Observed MW.	120 kDa
Subcellular Location	Membrane
Gene ID	56667
Swiss-Prot	Q9H3R2
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	MUC13 is a mucin. These are secreted or transmembrane glycoproteins that are expressed mainly in the digestive tract and on epithelial surfaces. MUC13 is upregulated in gastric cancer. Mucins have also been implicated in inflammatory bowel diseases.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human A549 cell lysates Primary: Anti-MUC13 (SL5877R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 120 kDa

Figure 2. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUC13) Polyclonal Antibody, Unconjugated (SL5877R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC13) Polyclonal Antibody, Unconjugated (SL5877R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.