Product Name	Rabbit Anti-NFKB2 Polyclonal Antibody
Cat No.	SLA0328
Chinese Name	细胞核因子/k基因结合核因子p100抗体
Alias	CVID10; H2TF1; LYT-10; LYT10; NF-kB2; p100; p49/p100; p52; NF-kappaB2; lyt; p49; p50B; RGD1307189; NFKB2_HUMAN; NFKB2; DNA-binding factor KBF2; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10 (Lyt10); NFKB2_MOUSE; nuclear factor kappa B subunit 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Clone No.
React Species	Human
Applications	WB (1:500-2000) Flow-Cyt (2ug/Test) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human NFKB2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	100 kDa
Observed MW.	52,120 kDa
Subcellular Location	Nucleus
Gene ID	4791
Swiss-Prot	Q00653
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human Daudi cell lysates Lane 4: Human MCF-7 cell lysates Primary: Anti-NFKB2 (SLA0328) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 52,120 kDa

Figure 2. Blank control (black line) :Hela. Primary Antibody (green line): Rabbit Anti-NFKB2 antibody (SLA0328) Dilution:2ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control (orange line) : Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed

Figure 3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NFKB2) polyclonal Antibody, Unconjugated (SLA0328) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.