Product Name	Recombinant Rabbit Anti-GFAP Monoclonal Antibody
Cat No.	SLA0525M
Chinese Name	胶质纤维酸性蛋白单克隆抗体
Alias	ALXDRD; GFAP_BOVIN; GFAP; GFAP_HUMAN; GFAP_MOUSE; glial fibrillary acidic protein; intermediate filament protein
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.	12C12
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human GFAP protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	48 kDa
Observed MW.	48 kDa
Subcellular Location	Cytoplasm
Gene ID	2670
Swiss-Prot	P14136
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (SLA0525M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(SLA0525M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(SLA0525M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.	Figure 4. (Negative control)Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(SLA0525M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.