Product Name	Recombinant Rabbit Anti-CA9 Monoclonal Antibody
Cat No.	SLA1342M
Chinese Name	碳酸酐酶9重组兔单抗
Alias	CAIX; MN; Ca9; MN/CA9; CAH9_HUMAN; Carbonate dehydratase IX; Carbonic anhydrase IX (CA-IX | CAIX); Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250 (RCC-associated antigen G250); pMW1; 4.2.1.1; G250; CAH9_MOUSE; Membrane antigen MN homolog; Car9; carbonic anhydrase 9; carbonic anhydrase IX; carbonic dehydratase; RCC-associated protein G250
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10A7
React Species	Human
Applications	IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Carbonic anhydrase 9
Purification	affinity purified by Protein A
Predicted MW.	50 kDa
Observed MW.	50 kDa
Subcellular Location	Nucleus
Gene ID	768
Swiss-Prot	Q16790
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated(SLA1342M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated(SLA1342M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CA9 | CAIX Antibody, Unconjugated (SLA1342M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human fundus of the stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CA9 | CAIX Antibody, Unconjugated (SLA1342M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.