Product Name	Recombinant Rabbit Anti-MPO Monoclonal Antibody
Cat No.	SLA0565M
Chinese Name	髓过氧化物酶单克隆抗体
Alias	mKIAA4033; PERM_HUMAN; MPO; 1.11.2.2; PERM_MOUSE; myeloperoxidase
Host Species	Mouse
Clonality	Monoclonal
Clone No.	6D9
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human MPO protein
Purification	affinity purified by Protein A
Predicted MW.	84 kDa
Observed MW.	84 kDa
Subcellular Location	Cytoplasm
Gene ID	4353
Swiss-Prot	P05164
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 50 ng rhMPO protein, C-His (HEK293) (SL43010P) per lane probed with MPO monoclonal antibody respectively, unconjugated (SLA0565M) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MPO Monoclonal Antibody, Unconjugated(SLA0565M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MPO Monoclonal Antibody, Unconjugated(SLA0565M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MPO Monoclonal Antibody, Unconjugated(SLA0565M) at 1:200 overnight at 4°C, followed by conjugation to the SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.