Product Name	Recombinant Rabbit Anti-TFRC Monoclonal Antibody
Cat No.	SLA0579M
Chinese Name	转铁蛋白受体（CD71）单克隆抗体
Alias	CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90; 2610028K12Rik; E430033M20Rik; Mtvr1; TFR1_HUMAN; TFRC; TFR1_MOUSE; TFR1_RAT; transferrin receptor; transferrin receptor (p90, CD71)
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	5D12
React Species	Human
Applications	WB (1:500-2000) Flow-Cyt (2ug/Test) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human CD71 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	85 kDa
Observed MW.	90 kDa
Subcellular Location	Membrane
Gene ID	7037
Swiss-Prot	P02786
Storage	Size : 50ul/100ul/200ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS (pH7.4). Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, unconjugated (SLA0579M) 1:200, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei

Figure 2. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HT-1080 cell lysates Primary: Anti-TFRC (SLA0579M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 74/85 kDa Observed band size: 90 kDa

Figure 3. Blank control:Hela. Primary Antibody (green line): Mouse Anti-TFRC antibody (SLA0579M) Dilution: 2ug/Test; Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC Dilution: 0.5ug/Test. Isotype control (orange line) : Normal Mouse IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed

Figure 4. HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (SLA0579M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.