Product Name	CALCA | Procalcitonin (PCT) Mouse mAb
Cat No.	SLM-41463M
Chinese Name	降钙素原单克隆抗体
Alias	CALC1; CGRP; CGRP-I; CGRP-alpha; CGRP1; CT; KC; PCT; CALC_HUMAN; CALCA; calcitonin related polypeptide alpha; calcitonin 1; calcitonin
Host Species	Mouse
Isotype	IgG
Clonality	Monoclonal
Clone No.	2F2
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human Procalcitonin(PCT)
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	13 kDa
Observed MW.	17 kDa
Subcellular Location	Secreted
Gene ID	796
Swiss-Prot	P01258
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Sample: Lane 1: Recombinant human Procalcitonin, N-His Primary: Anti-Procalcitonin (PCT) (SLM-41463M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 13 kDa Observed band size: 17 kDa

Figure 2. Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Procalcitonin (PCT) ) Monoclonal Antibody, Unconjugated (SLM-41463M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Procalcitonin (PCT) Monoclonal Antibody, Unconjugated (SLM-41463M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, SL0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Figure 4. 4% Paraformaldehyde-fixed TT(treated with BFA)(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Procalcitonin(PCT)) monoclonal Antibody, unconjugated (SLM-41463M) 1:100, 90 min at 37°C; followed by FITC conjugated Goat Anti-Mouse IgG antibody (green, SL60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.