Product Name	PMS2 Recombinant Rabbit mAb
Cat No.	SLM-55559R
Chinese Name	肿瘤错配修复基因PMS2重组兔单抗
Alias	HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2; PMS2CL; PMSL2; PMS2_HUMAN; PMS2; DNA mismatch repair protein PMS2; PMS1 protein homolog 2; 3.1.-.-; PMS2_MOUSE; PMS1 homolog 2, mismatch repair system component; postmeiotic segregation increased (S. cerevisiae) 2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMS1 homolog 2, mismatch repair protein
Host Species	Rabbit
Isotype	IgG
Clonality	Recombinant
Clone No.	5F9
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human PMS2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	103 kDa
Observed MW.	105 kDa
Subcellular Location	Nucleus
Gene ID	5395
Swiss-Prot	P54278
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PMS2 monoclonal antibody, unconjugated (SLM-55559R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PMS2 Monoclonal Antibody, Unconjugated(SLM-55559R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PMS2 Monoclonal Antibody, Unconjugated(SLM-55559R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLM-55559R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PMS2 antibody (SLM-55559R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.