Product Name	Recombinant Rabbit Anti-PROM1 Monoclonal Antibody
Cat No.	SLA2405M
Chinese Name	造血干细胞抗原CD133重组兔单抗
Alias	AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4; 4932416E19Rik; Prom; Prom-1; PROM1_HUMAN; PROM1; Antigen AC133; Prominin-like protein 1; PROM1_MOUSE; Antigen AC133 homolog; prominin 1; prominin (mouse)-like 1; macular dystrophy, retinal 2; Stargardt disease 4 (autosomal dominant); AC133 antigen; Retinitis pigmentosa 41, Cone-rod dystrophy 12
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11G1
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:200-1000) IHC-F (1:200-1000) IF (1:200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CD133
Purification	affinity purified by Protein A
Predicted MW.	97 kDa
Observed MW.	130 kDa
Subcellular Location	Membrane
Gene ID	8842
Swiss-Prot	O43490
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CD133 monoclonal antibody, unconjugated (SLA2405M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (SLA2405M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (SLA2405M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (SLA2405M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (SLA2405M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.